Tools

Pipette-Guide-96 – A tablet ap for enabling quick picking or adding of different volumes to wells of a microtiter plate (also works on smartphones). Start from the highest volume and go down. Takes less than 20 minutes to take different volumes from 96 wells into a single tube (e.g. pool genomic libraries for sequencing). Faster than any robot I’ve worked with.

 

Protocols

$8 genomic library preparation – A modified Nextera protocol that makes Illumina sample prep for a bacterial genome less expensive sequencing. $8 to go from gDNA to QC’d libraries. Main innovations are smaller tagmentation reaction volume and cheaper reagents. Easy to adopt and modify.